Microcapsules of Poly(3-Hydroxybutyrate) for Sustained Protein Release

被引：5

作者：

Zernov, A. L. ^{[1
,3
]}

Ivanov, E. A. ^{[1
]}

Makhina, T. K. ^{[1
]}

Myshkina, V. L. ^{[1
]}

Samsonova, O. V. ^{[5
]}

Feofanov, A. V. ^{[5
]}

Volkov, A. V. ^{[7
]}

Gazhva, J. V. ^{[3
,4
]}

Muraev, A. A. ^{[3
,4
,8
]}

Ryabova, V. M. ^{[3
,4
]}

Shaitan, K. V. ^{[5
,6
]}

Ivanov, S. Y. ^{[3
,4
,8
]}

Bonartseva, G. A. ^{[1
]}

Bonartsev, A. P. ^{[2
,3
,5
]}

机构：

[1] Russian Acad Sci, Fed Res Ctr Fundamentals Biotechnol, AN Bach Inst Biochem, Lab Microorganism Nitrogen Fixat, 33,Bld 2 Leninsky Prospect, Moscow 119071, Russia

[2] Russian Acad Sci, Fed Res Ctr Fundamentals Biotechnol, AN Bach Inst Biochem, Moscow 119071, Russia

[3] Nizhny Novgorod State Med Acad, Nizhnii Novgorod 603005, Russia

[4] Nizhny Novgorod State Med Acad, Dept Maxillaofacial Surg & Implantol, Nizhnii Novgorod 603005, Russia

[5] Moscow MV Lomonosov State Univ, Dept Bioengn, Moscow 119991, Russia

[6] Moscow MV Lomonosov State Univ, Moscow 119991, Russia

[7] Minist Hlth Russian Federat, Cent Res Inst Dent & Maxillofacial Surg, Dept Gen Pathol, Moscow 119991, Russia

[8] Peoples Friendship Univ Russia, Dept Maxillofacial Surg & Surg Dent, Moscow 117198, Russia

来源：

SOVREMENNYE TEHNOLOGII V MEDICINE | 2015年 / 7卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

biodegradable polymers; polyhydroxyalkanoates; poly(3-hydroxybutyrate); prolonged release; microencapsulation; biocompatibility;

D O I：

10.17691/stm2015.7.4.06

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The aim of investigation was to develop a system for protein sustained release based on the use of poly(3-hydroxybutyrate) (PHB) microcapsules loaded with bovine serum albumin (BSA). Materials and Methods. To develop microcapsule we used PHB obtained microbiologically by a strain-producer Azotobacter chroococcum 7B. Microcapsules loaded with model protein BSA were produced by double emulsion technique "water/oil/water". Morphology of microcapsules was investigated by methods of confocal and scanning electron microscopy, when the loading and release of BSA was examined spectrophotometrically. In vivo biocompatibility of microcapsules was studied in accordance with intramuscular implantation and histology findings. Results. The study of BSA incorporation and its sustained release from microcapsules for more than 190 h demonstrated the efficacy of proposed system. The mechanism of protein release was found to occur due to the rupture of polymer walls. Moderate tissue response to the implantation of obtained microcapsules was demonstrated. Conclusion. Developed PHB microcapsules loaded with BSA are good model of long acting protein drugs.

引用

页码：50 / 56

页数：7

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