A CONVENIENT PROCEDURE FOR PREPARING TRANSFER RIBONUCLEIC ACID FROM ESCHERICHIA COLI

被引：82

作者：

AVITAL, S

ELSON, D

机构：

[1] Biochemistry Department, The Weizmann Institute of Science, Rehovoth

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 179卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0005-2787(69)90038-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for the isolation of transfer RNA (tRNA) from Escherichia coli. The method is a combination of several known techniques. tRNA is extracted from whole cells with phenol, stripped of attached amino acids at pH 8, preferentially solubilized with 2 M LiCl, and reprecipitated with (NH4)2SO4. The key step is the use of 2 M LiCl, which separates ribosomal RNA from tRNA rapidly and completely. The method is simple and yields tRNA as active as the best preparations that we have obtained with other methods. © 1969.

引用

页码：297 / &

共 30 条

[1] PURIFICATION AND PROPERTIES OF POLIOVIRUS DOUBLE-STRANDED RIBONUCLEIC ACID [J].