MOLECULAR-CLONING OF A NOVEL PUTATIVE G-PROTEIN-COUPLED RECEPTOR EXPRESSED IN THE CARDIOVASCULAR-SYSTEM

被引：134
作者：
OKAZAKI, H
ISHIZAKA, N
SAKURAI, T
KUROKAWA, K
GOTO, K
KUMADA, M
TAKUWA, Y
机构：
[1] UNIV TOKYO,FAC MED,DEPT CARDIOVASC BIOL,7-3-1 HONGO,BUNKYO KU,TOKYO 113,JAPAN
[2] UNIV TOKYO,FAC MED,DEPT INTERNAL MED,TOKYO 113,JAPAN
[3] UNIV TOKYO,FAC MED,DEPT PHYSIOL,TOKYO 113,JAPAN
[4] UNIV TSUKUBA,INST BASIC MED SCI,DEPT PHARMACOL,TSUKUBA,IBARAKI 305,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 190卷 / 03期
关键词：
D O I：
10.1006/bbrc.1993.1163
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel cDNA clone encoding a putative G protein-coupled receptor has been isolated from a rat aortic smooth muscle cDNA library. Sequence analysis of this clone reveals the structural features characteristic of this superfamily, with a high degree of sequence similarity to the Edg-1 receptor previously reported to be expressed in vascular endothelial cells. This novel receptor mRNA is expressed predominantly in the lung and heart. Although the ligand for this receptor has yet to be identified, the results suggest the existence of a new receptor subfamily in the cardiovascular system thus far unrecognized. © 1993 Academic Press, Inc.
引用
收藏
页码：1104 / 1109
页数：6
相关论文
共 10 条
[1]   SMOOTH-MUSCLE CELL IN CULTURE [J].
CHAMLEYCAMPBELL, J ;
CAMPBELL, GR ;
ROSS, R .
PHYSIOLOGICAL REVIEWS, 1979, 59 (01) :1-61
[2]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[3]   MODEL SYSTEMS FOR THE STUDY OF 7-TRANSMEMBRANE-SEGMENT RECEPTORS [J].
DOHLMAN, HG ;
THORNER, J ;
CARON, MG ;
LEFKOWITZ, RJ .
ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :653-688
[4]  
HLA T, 1990, J BIOL CHEM, V265, P9308
[5]  
KENNELLY PJ, 1991, J BIOL CHEM, V266, P1555
[6]  
KOZAK M, 1983, NUCLEIC ACIDS RES, V12, P865
[7]   A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].
KYTE, J ;
DOOLITTLE, RF .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132
[8]   SELECTIVE AMPLIFICATION AND CLONING OF 4 NEW MEMBERS OF THE G-PROTEIN COUPLED RECEPTOR FAMILY [J].
LIBERT, F ;
PARMENTIER, M ;
LEFORT, A ;
DINSART, C ;
VANSANDE, J ;
MAENHAUT, C ;
SIMONS, MJ ;
DUMONT, JE ;
VASSART, G .
SCIENCE, 1989, 244 (4904) :569-572
[9]   STRUCTURE-FUNCTION RELATIONSHIP OF PROTEINS BELONGING TO THE FAMILY OF RECEPTORS COUPLED TO GTP-BINDING PROTEINS [J].
STROSBERG, AD .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 196 (01) :1-10
[10]   SUBSTRATE-SPECIFICITY OF PROTEIN-KINASE-C - USE OF SYNTHETIC PEPTIDES CORRESPONDING TO PHYSIOLOGICAL SITES AS PROBES FOR SUBSTRATE RECOGNITION REQUIREMENTS [J].
WOODGETT, JR ;
GOULD, KL ;
HUNTER, T .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 161 (01) :177-184
1