Little is known about the effects of interferon (IFN) on cell function in the eye. We have analyzed the effect of INF-alpha and IFN-gamma on the expression of proteins in cultured human corneal fibroblasts. Treatment with IFN-alpha increased the synthesis of proteins of 84, 76, 52, and 28 kD and decreased the synthesis of a 72-kD protein. Treatment with IFN-gamma increased the synthesis of proteins of 83, 66, 64, 54, and 47 kD. The effect of IFN-alpha and IFN-gamma were first detected at 5-9 h and 9 h, respectively, after the addition of the IFNs and were maximal at 17 and 24 h, respectively. Most of the changes were seen at doses of 1 x 10(1) to 1 x 10(2) U/ml of IFN-alpha or IFN-gamma and were maximal at 1 x 10(2) to 1 x 10(3) U/ml. Thus, each IFN induced distinct proteins based on apparent molecular weight and isoelectric point. These results show that IFN-alpha and IFN-gamma affect the synthesis of small groups of distinct proteins in human corneal fibroblasts.
机构:
MEM SLOAN KETTERING CANC CTR, GRAD PROGRAM MOLEC BIOL, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, GRAD PROGRAM MOLEC BIOL, NEW YORK, NY 10021 USA
CAPLEN, HS
GUPTA, SL
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机构:
MEM SLOAN KETTERING CANC CTR, GRAD PROGRAM MOLEC BIOL, NEW YORK, NY 10021 USAMEM SLOAN KETTERING CANC CTR, GRAD PROGRAM MOLEC BIOL, NEW YORK, NY 10021 USA