PURIFICATION AND CHARACTERIZATION OF 2 DISTINCT LIPASES FROM CANDIDA-CYLINDRACEA

被引：209
作者：
RUA, ML [1 ]
DIAZMAURINO, T [1 ]
FERNANDEZ, VM [1 ]
OTERO, C [1 ]
BALLESTEROS, A [1 ]
机构：
[1] CSIC,INST QUIM FIS ROCASOLANO,MADRID 6,SPAIN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1156卷 / 02期
关键词：
LIPASE; ISOENZYME; ENZYME PURIFICATION; ENZYME CHARACTERIZATION; CANDIDA-CYLINDRACEA; CANDIDA-RUGOSA;
D O I：
10.1016/0304-4165(93)90134-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have purified and characterized two isoenzymes from a commercial lipase preparation of Candida cylindracea. The purification procedure includes ethanol precipitation and DEAE Sephacel and Sephacryl HR 100 chromatographies. Lipase A and lipase B were purified 11-fold with a 5% and 21% recovery in activity, respectively. The enzymes have similar amino acid content, N-terminal sequence and molecular weight, but differ on neutral sugar content, hydrophobicity, presence of isoforms and stability to pH and temperature. They also show some differences in the substrate specificity.
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页码：181 / 189
页数：9
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