AUTOMETALLOGRAPHY - TISSUE METALS DEMONSTRATED BY A SILVER ENHANCEMENT KIT

被引:65
作者
DANSCHER, G
NORGAARD, JOR
BAATRUP, E
机构
[1] Institute of Anatomy, University of Aarhus, Aarhus C
关键词
D O I
10.1007/BF00500618
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In biological tissue, minute accumulations of gold, silver, mercury and zinc can be visualized by a technique whereby metallic silver is precipitated on tiny accumulations of the two noble metals, or on selenites or sulphides of all four metals. In the present study a silver enhancement kit, primarily intended for the amplification of colloidal gold particles, has been used to demonstrate these catalytic tissue metals. Sections from animals exposed intravitally aurothiomalatate, silver lactate, mercury chloride, sodium selenite or perfused with sodium sulphide were subjected to a commercial silver enhancement kit (IntenSE, Janssen Pharmaceutica). It was found that the kit performs adequately to the silver lactate gum arabic developer and to the photographic emulsion technique. The kit can be used as a silver enhancement medium for the demonstration of zinc by the Neo Timm and selenium methods and for demonstration of gold, silver, and mercury in tissues from animals intravitally exposed to these metals. It can also be used for counterstaining silver treated osmium fixed tissues embedded in plastic. © 1987 Springer-Verlag.
引用
收藏
页码:465 / 469
页数:5
相关论文
共 14 条
[1]   SILVER AMPLIFICATION OF MERCURY SULFIDE AND SELENIDE - A HISTOCHEMICAL METHOD FOR LIGHT AND ELECTRON-MICROSCOPIC LOCALIZATION OF MERCURY IN TISSUE [J].
DANSCHER, G ;
MOLLERMADSEN, B .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1985, 33 (03) :219-228
[2]   LIGHT AND ELECTRON-MICROSCOPIC LOCALIZATION OF SILVER IN BIOLOGICAL TISSUE [J].
DANSCHER, G .
HISTOCHEMISTRY, 1981, 71 (02) :177-186
[4]   LIGHT MICROSCOPIC VISUALIZATION OF COLLOIDAL GOLD ON RESIN-EMBEDDED TISSUE [J].
DANSCHER, G ;
NORGAARD, JOR .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1983, 31 (12) :1394-1398
[6]   THE DITHIZONE, TIMM SULFIDE SILVER AND THE SELENIUM METHODS DEMONSTRATE A CHELATABLE POOL OF ZINC IN CNS - A PROTON ACTIVATION (PIXE) ANALYSIS OF CARBON-TETRACHLORIDE EXTRACTS FROM RAT BRAINS AND SPINAL CORDS INTRAVITALLY TREATED WITH DITHIZONE [J].
DANSCHER, G ;
HOWELL, G ;
PEREZCLAUSELL, J ;
HERTEL, N .
HISTOCHEMISTRY, 1985, 83 (05) :419-422
[9]   A SILVER METHOD FOR COUNTERSTAINING PLASTIC EMBEDDED TISSUE [J].
DANSCHER, G .
STAIN TECHNOLOGY, 1983, 58 (06) :365-372
[10]  
DANSCHER G, 1985, J HISTOCHEM CYTOCHEM, V33, P705