EXPRESSION CLONING OF A RAT GLUTAMATE TRANSPORTER

被引：109

作者：

TANAKA, K

机构：

[1] Laboratory for Neural Network, Frontier Research Program, The Institute of Physical and Chemichal Research (RIKEN), Wako, Saitama

来源：

NEUROSCIENCE RESEARCH | 1993年 / 16卷 / 02期

关键词：

GLUTAMATE TRANSPORTER; XENOPUS OOCYTE; RAT BRAIN; EXPRESSION CLONING;

D O I：

10.1016/0168-0102(93)90082-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A functional cDNA clone for the rat glutamate transporter was isolated by a cloning approach using a Xenopus oocyte expression system. The cDNA sequence predicts a protein of 543 amino acids with 6 putative transmembrane domains. The glutamate transporter shows no sequence similarity to other members of the known neurotransmitter transporter family. Expression of the cDNA clone in Xenopus oocyte indicates that glutamate transport activity is Na+- and Cl--dependent and sensitive to selective glutamate transporter inhibitor. The glutamate transporter mRNA is expressed in both the brain and the peripheral tissues at different levels.

引用

页码：149 / 153

页数：5

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