DIRECT VISUALIZATION OF SUPERCOILED DNA-MOLECULES IN SOLUTION

被引：139

作者：

ADRIAN, M ^{[1
]}

TENHEGGELERBORDIER, B ^{[1
]}

WAHLI, W ^{[1
]}

STASIAK, AZ ^{[1
]}

STASIAK, A ^{[1
]}

DUBOCHET, J ^{[1
]}

机构：

[1] UNIV LAUSANNE,INST BIOL ANIM,BATIMENT BIOL,CH-1015 LAUSANNE,SWITZERLAND

来源：

EMBO JOURNAL | 1990年 / 9卷 / 13期

关键词：

CRYOELECTRON MICROSCOPY; DNA STRUCTURE; DNA TOPOLOGY; INTERWOUND SUPERCOILING; SUPERCOILED DNA;

D O I：

10.1002/j.1460-2075.1990.tb07907.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The shape of supercoiled DNA molecules in solution is directly visualized by cryo-electron microscopy of vitrified samples. We observe that: (i) supercoiled DNA molecules in solution adopt an interwound rather than a toroidal form, (ii) the diameter of the interwound superhelix changes from about 12 nm to 4 nm upon addition of magnesium salt to the solution and (iii) the partition of the linking deficit between twist and writhe can be quantitatively determined for individual molecules.

引用

页码：4551 / 4554

页数：4

共 18 条

[1] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].

ADRIAN, M ;

DUBOCHET, J ;

LEPAULT, J ;

MCDOWALL, AW .

NATURE, 1984, 308 (5954) :32-36

[2]

BAUER WR, 1980, SCI AM, V243, P118

[3] USE OF SITE-SPECIFIC RECOMBINATION AS A PROBE OF DNA-STRUCTURE AND METABOLISM INVIVO [J].

BLISKA, JB ;

COZZARELLI, NR .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (02) :205-218

[4] STRUCTURE OF PLECTONEMICALLY SUPERCOILED DNA [J].

BOLES, TC ;

WHITE, JH ;

COZZARELLI, NR .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) :931-951

[5] X-RAY-DIFFRACTION STUDIES OF CIRCULAR SUPERHELICAL DNA AT 300-10,000-A RESOLUTION [J].

BRADY, GW ;

FEIN, DB ;

BRUMBERGER, H .

NATURE, 1976, 264 (5583) :231-234

[6] ENVIRONMENTAL-INFLUENCES ON DNA SUPERHELICITY - THE EFFECT OF IONIC-STRENGTH ON SUPERHELIX CONFORMATION IN SOLUTION [J].

BRADY, GW ;

SATKOWSKI, M ;

FOOS, D ;

BENHAM, CJ .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 195 (01) :185-191

[7]

CYRKLAFF M, IN PRESS J ULTRASTRU

[8] CRYO-ELECTRON MICROSCOPY OF VITRIFIED BIOLOGICAL SPECIMENS [J].

DUBOCHET, J ;

ADRIAN, M ;

LEPAULT, J ;

MCDOWALL, AW .

TRENDS IN BIOCHEMICAL SCIENCES, 1985, 10 (04) :143-146

[9] CRYO-ELECTRON MICROSCOPY OF VITRIFIED SPECIMENS [J].

DUBOCHET, J ;

ADRIAN, M ;

CHANG, JJ ;

HOMO, JC ;

LEPAULT, J ;

MCDOWALL, AW ;

SCHULTZ, P .

QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (02) :129-228

[10] TORSIONAL RIGIDITY OF DNA AND LENGTH DEPENDENCE OF THE FREE-ENERGY OF DNA SUPERCOILING [J].

HOROWITZ, DS ;

WANG, JC .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 173 (01) :75-91

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