INDUCTION OF THE HIGH-AFFINITY NA+-DEPENDENT GLUTAMATE TRANSPORT-SYSTEM X(AG)- BY HYPERTONIC STRESS IN THE RENAL EPITHELIAL-CELL LINE NBL-1

被引：22

作者：

FERRERMARTINEZ, A

FELIPE, A

NICHOLSON, B

CASADO, J

PASTORANGLADA, M

MCGIVAN, J

机构：

[1] UNIV BRISTOL,SCH MED SCI,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

[2] UNIV BARCELONA,DEPT BIOQUIM & FISIOL,UNITATA BIOQUIM & BIOL MOLEC,E-08028 BARCELONA,SPAIN

来源：

BIOCHEMICAL JOURNAL | 1995年 / 310卷

关键词：

D O I：

10.1042/bj3100689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The high-affinity Na+-dependent glutamate transport system X(AG-) is induced (threefold increase in V-max with no change in K-m) by hypertonicity in the renal epithelial cell line NBL-1. This effect is dependent on protein synthesis and glycosylation and is accompanied by an increase in EAAC1 mRNA levels. Other Na+-dependent transport systems in this cell line do not respond to hypertonic stress. In contrast to recent findings [Ruiz-Montasell, Gomez-Angelats, Casado, Felipe, McGivan and Pastor-Anglada (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 9569-9573] showing that increased system A activity after hyperosmotic shock results from induction of a regulatory protein, this is the first demonstration that hypertonicity may increase the expression of the gene for an amino acid transport protein itself.

引用

页码：689 / 692

页数：4

共 15 条

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