CHANGES IN SPHINGOSINE CONTENT IN REGENERATING RAT-LIVER CELL-NUCLEI

被引：0

作者：

ALESENKO, AV

PANTAZ, EA

PUSHKAREVA, MY

RUSAKOV, SA

FILIPPOVA, GN

机构：

来源：

BIOCHEMISTRY-MOSCOW | 1993年 / 58卷 / 03期

关键词：

SPHINGOSINE; SPHINGOMYELIN CYCLE; NUCLEUS; DNA AND RNA SYNTHESIS; REGENERATING LIVER;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sphingosine content in regenerating rat liver cell nuclei was analyzed by high-performance liquid chromatography during RNA and DNA synthesis. Experimental activation of nucleic acid synthesis induced sphingosine accumulation in the cell nuclei. In parallel, induction of the sphingomyelin cycle was observed, involving the activation of sphingomyelinase and changes in sphingomyelin and ceramide contents. The interaction of sphingosine with DNA and the influence of sphingosine on RNA polymerases were studied in vitro to clarify the role of sphingosine in replication and transcription. At a concentration of 10(-4) M, sphingosine hindered the interaction of acridine orange with DNA and activated transcription enzymes. Several alternative mechanisms for the involvement of sphingosine in the regulation of nucleic acid synthesis are discussed.

引用

页码：298 / 305

页数：8

共 23 条

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[2]

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