A HIGHLY REPETITIVE DNA-SEQUENCE POSSIBLY UNIQUE TO CANIDS

被引：59

作者：

MINNICK, MF ^{[1
]}

STILLWELL, LC ^{[1
]}

HEINEMAN, JM ^{[1
]}

STIEGLER, GL ^{[1
]}

机构：

[1] PACIFIC NW LAB, DEPT BIOL, RICHLAND, WA 99352 USA

来源：

GENE | 1992年 / 110卷 / 02期

关键词：

CANIS-FAMILIARIS; DOG; REPETITIVE ELEMENT; NUCLEOTIDE SEQUENCE; SHORT INTERSPERSED NUCLEOTIDE ELEMENT; INTERFERON;

D O I：

10.1016/0378-1119(92)90654-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A short interspersed nucleotide (nt) element (SINE) was cloned from the genomic DNA of the domestic dog, Canis familiaris. Southern-blot analysis of canine DNA digested with four restriction endonucleases indicated that the SINE is widely dispersed throughout the genome. Hybridizations also indicated that the element may be unique to canids and is absent in a variety of other mammals, including members of four closely-related carnivore families. Three examples of the SINE have been located and sequenced. The 130-bp SINE contains putative RNA polymerase III transcriptional control sequences. The SINE is flanked at the 3' end by a (TC)8-repeat region followed by a poly(A) tract of 35-65 nt. Computer database searches located two homologous sequences with approx. 80% identity to the SINE. These sequences were located in untranslated regions of the canine genes encoding interferon-omega and clotting factor IX.

引用

页码：235 / 238

页数：4

共 21 条

[1] PHENOTYPIC CORRECTION OF FACTOR-IX DEFICIENCY IN SKIN FIBROBLASTS OF HEMOPHILIC DOGS [J].