CELLULAR AND SUBCELLULAR-LOCALIZATION OF THE MGLUR5A METABOTROPIC GLUTAMATE-RECEPTOR IN RAT SPINAL-CORD

被引：96
作者：
VIDNYANSZKY, Z
HAMORI, J
NEGYESSY, L
RUEGG, D
KNOPFEL, T
KUHN, R
GORCS, TJ
机构：
[1] HUNGARIAN ACAD SCI,UNITED RES ORG,NEUROBIOL LAB,H-1094 BUDAPEST,HUNGARY
[2] SEMMELWEIS UNIV,SCH MED,H-1094 BUDAPEST,HUNGARY
[3] CIBA GEIGY AG,CNS RES,DEPT MOLEC & CELLULAR BIOL,CH-4002 BASEL,SWITZERLAND
来源：
NEUROREPORT | 1994年 / 6卷 / 01期
关键词：
DORSAL HORN; IMMUNOHISTOCHEMISTRY; ANTIBODY; NOCICEPTION; SYNAPTIC TRANSMISSION;
D O I：
10.1097/00001756-199412300-00053
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
THE cellular and subcellular distribution of the mGluR5a metabotropic glutamate receptor was studied in the spinal cord of the rat using an antibody raised against a mGluR5a-specific carboxy-terminal peptide. Strong mGluR5a-immunoreactivity (mGluRSa-ir) was found in the laminae I-II of the dorsal horn, which gradually decreased towards the deeper layers. At the electron microscopical level, mGluR5a-ir was present exclusively in neuronal somata and dendrites. Immunometal labelling revealed that mGluR5a-ir is concentrated at the periphery of postsynaptic densities of asymmetrical synapses or localized extrasynaptically at dendritic and somatic membranes. The mGluR5a-immunoreactive dendritic profiles were often targeted by synaptic boutons with the morphological characteristics of C-fibre terminals. These observations provide evidence for mGluR5a being involved in the nociceptive transmission at the dorsal horn.
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页码：209 / 213
页数：5
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