NMR DETERMINATION OF RESIDUAL STRUCTURE IN A UREA-DENATURED PROTEIN, THE 434-REPRESSOR

被引：482

作者：

NERI, D ^{[1
]}

BILLETER, M ^{[1
]}

WIDER, G ^{[1
]}

WUTHRICH, K ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,INST MOLEK BIOL & BIOPHYS,CH-8092 ZURICH,SWITZERLAND

来源：

SCIENCE | 1992年 / 257卷 / 5076期

关键词：

D O I：

10.1126/science.1523410

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A nuclear magnetic resonance (NMR) structure determination is reported for the polypeptide chain of a globular protein in strongly denaturing solution. Nuclear Overhauser effect (NOE) measurements with a 7 molar urea solution of the amino-terminal 63-residue domain of the 434-repressor and distance geometry calculations showed that the polypeptide segment 54 to 59 forms a hydrophobic cluster containing the side chains of Val54, Val56, Trp58, and Leu59. This residual structure in the urea-unfolded protein is related to the corresponding region of the native, folded protein by simple rearrangements of the residues 58 to 60. Based on these observations a model for the early phase of refolding of the 434-repressor(1-63) is proposed.

引用

页码：1559 / 1563

页数：5

共 28 条

[21] STRUCTURAL CHARACTERIZATION OF FOLDING INTERMEDIATES IN CYTOCHROME-C BY H-EXCHANGE LABELING AND PROTON NMR [J].

RODER, H ;

ELOVE, GA ;

ENGLANDER, SW .

NATURE, 1988, 335 (6192) :700-704

[22] STEREOSPECIFIC ASSIGNMENT OF THE METHYL H-1-NMR LINES OF VALINE AND LEUCINE IN POLYPEPTIDES BY NONRANDOM C-13 LABELING [J].

SENN, H ;

WERNER, B ;

MESSERLE, BA ;

WEBER, C ;

TRABER, R ;

WUTHRICH, K .

FEBS LETTERS, 1989, 249 (01) :113-118

[23]

SINGH UC, 1986, AMBER30 U CAL

[24] NMR EVIDENCE FOR AN EARLY FRAMEWORK INTERMEDIATE ON THE FOLDING PATHWAY OF RIBONUCLEASE-A [J].

UDGAONKAR, JB ;

BALDWIN, RL .

NATURE, 1988, 335 (6192) :694-699

[25] PROTON PROTON OVERHAUSER EFFECTS OF RECEPTOR-BOUND CYCLOSPORINE-A OBSERVED WITH THE USE OF A HETERONUCLEAR-RESOLVED HALF-FILTER EXPERIMENT [J].

WIDER, G ;

WEBER, C ;

WUTHRICH, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (12) :4676-4678

[26] PROTEIN-STRUCTURE DETERMINATION IN SOLUTION BY NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY [J].

WUTHRICH, K .

SCIENCE, 1989, 243 (4887) :45-50

[27] PSEUDO-STRUCTURES FOR THE 20 COMMON AMINO-ACIDS FOR USE IN STUDIES OF PROTEIN CONFORMATIONS BY MEASUREMENTS OF INTRAMOLECULAR PROTON PROTON DISTANCE CONSTRAINTS WITH NUCLEAR MAGNETIC-RESONANCE [J].

WUTHRICH, K ;

BILLETER, M ;

BRAUN, W .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 169 (04) :949-961

[28]

Wuthrich K., 1986, NMR PROTEINS NUCLEIC

← 1 2 3 →