SYNTHESIS OF BACKBONE DEUTERIUM LABELED [R(CGCGAA-UUCGCG)]2 AND HPLC PURIFICATION OF SYNTHETIC RNA

被引：12

作者：

KHARE, D ^{[1
]}

ORBAN, J ^{[1
]}

机构：

[1] UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR ADV RES BIOTECHNOL,9600 GUDELSKY DR,ROCKVILLE,MD 20850

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 19期

关键词：

D O I：

10.1093/nar/20.19.5131

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical synthesis of backbone deuterium labelled [r(CGCGAAU*U*CGCG)]2 (U* = [5'-H-2]U) is described. An efficient purification procedure was developed using a polymeric reverse phase (PRP) HPLC column at 60-degrees-C. This procedure provided pure RNA dodecamer in the multi-milligram quantities (39% overall yield) necessary for dynamics studies using solid-state deuterium NMR. The purification method has been effectively applied to other RNA sequences and will assist biophysical studies which require relatively large quantities of RNA oligomers.

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页码：5131 / 5136

页数：6

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