COMPARATIVE-ANALYSIS OF BACILLUS-ANTHRACIS, BACILLUS-CEREUS, AND RELATED SPECIES ON THE BASIS OF REVERSE-TRANSCRIPTASE SEQUENCING OF 16S RIBOSOMAL-RNA

被引：372
作者：
ASH, C
FARROW, JAE
DORSCH, M
STACKEBRANDT, E
COLLINS, MD
机构：
[1] INST FOOD RES, DEPT MICROBIOL, READING RG2 9AT, ENGLAND
[2] UNIV QUEENSLAND, DEPT MICROBIOL, BRISBANE, QLD 4072, AUSTRALIA
来源：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1991年 / 41卷 / 03期
关键词：
RIBOSOMAL-RNA; GEN-NOV;
D O I：
10.1099/00207713-41-3-343
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The primary structures of the 16S rRNAs of Bacillus anthracis, Bacillus cereus, Bacillus mycoides, and Bacillus thuringiensis were determined by using the reverse transcription-dideoxy sequencing method. All of the strains exhibited very high levels of sequence similarity (> 99%) that were consistent with the close relationships shown by previous DNA hybridization studies. The sequences of B. anthracis Sterne and B. cereus emetic strain NCTC 11143 were found to be identical for a continuous stretch of 1,446 bases and differed from the sequence of B. cereus NCDO 1771T (T = type strain) by only a single nucleotide. The 16S rRNA sequences of B. mycoides and B. thuringiensis differed from each other and from the sequences of B. anthracis and B. cereus by four to nine nucleotides.
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页码：343 / 346
页数：4
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