METABOLISM OF ESTROGENS INVIVO AND INVITRO BY PHASEOLUS-VULGARIS

被引：11
|
作者：
YOUNG, IJ [1 ]
KNIGHTS, BA [1 ]
HILLMAN, JR [1 ]
机构：
[1] UNIV GLASGOW, DEPT BOT, GLASGOW G12 8QQ, SCOTLAND
来源：
ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE | 1979年 / 94卷 / 04期
关键词：
D O I：
10.1016/S0044-328X(79)80114-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Seedlings of P. vulgaris cv. Canadian Wonder were incubated with 4-14C-estrone, 4-14C-estradiol-17.beta. and 6,7-3H-estrone sulphate. Evidence from TLC, column chromatography on Lipidex, radio-GLC and crystallization to constant specific activity suggests that estrone and estradiol-17.beta. are interconvertible in plant tissues. A tissue homogenate supplemented with cofactors evidently can metabolize estrone. Analysis by gas chromatography and combined gas chromatography-mass spectrometry demonstrated unequivocally that the major product was estradiol. These data represent the 1st evidence from full mass spectrometry that estrogens can be metabolized by plant tissues.
引用
收藏
页码：307 / 316
页数：10
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