METABOLISM OF ESTROGENS INVIVO AND INVITRO BY PHASEOLUS-VULGARIS

被引：11
|
作者：
YOUNG, IJ [1 ]
KNIGHTS, BA [1 ]
HILLMAN, JR [1 ]
机构：
[1] UNIV GLASGOW, DEPT BOT, GLASGOW G12 8QQ, SCOTLAND
来源：
ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE | 1979年 / 94卷 / 04期
关键词：
D O I：
10.1016/S0044-328X(79)80114-2
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Seedlings of P. vulgaris cv. Canadian Wonder were incubated with 4-14C-estrone, 4-14C-estradiol-17.beta. and 6,7-3H-estrone sulphate. Evidence from TLC, column chromatography on Lipidex, radio-GLC and crystallization to constant specific activity suggests that estrone and estradiol-17.beta. are interconvertible in plant tissues. A tissue homogenate supplemented with cofactors evidently can metabolize estrone. Analysis by gas chromatography and combined gas chromatography-mass spectrometry demonstrated unequivocally that the major product was estradiol. These data represent the 1st evidence from full mass spectrometry that estrogens can be metabolized by plant tissues.
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页码：307 / 316
页数：10
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