ILVEDA OPERON OF ESCHERICHIA-COLI-K12 ENCODES ONLY ONE VALINE-ALPHA-KETOGLUTARATE TRANSAMINASE-ACTIVITY

被引：9

作者：

ADAMS, CW

LAWTHER, RP

HATFIELD, GW

机构：

[1] Department of Microbiology College, Medicine University of California, Irvine, Irvine

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(79)90679-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transaminase B of E. coli K12 was purified to apparent homogeneity as measured by SDS acrylamide gel electrophoresis, immunoelectrophoresis, and amino terminal sequence analysis. The valine- and isoleucine-α-ketoglutarate dependent transaminase activities of pure enzyme as well as crude extracts were characterized by immunologic and kinetic methods. The data disprove the existence of a separate valine-α-ketoglutarate transaminase within the ilvEDA operon. © 1979.

引用

收藏

页码：650 / 658

页数：9

相关论文

共 25 条

[1]

BERG CM, M GENETICS

[2] TRANSAMINATION OF L-ASPARTATE AND L-PHENYLALANINE IN ESCHERICHIA-COLI K-12 [J].

CHESNE, S ;

MONTMITONNET, A ;

PELMONT, J .

BIOCHIMIE, 1975, 57 (09) :1029-1034

[3]

COHEN BM, 1976, GENETICS, V83, P201

[4] NONIDENTITY OF ASPARTATE AND AROMATIC AMINOTRANSFERASE COMPONENTS OF TRANSAMINASE-A IN ESCHERICHIA-COLI [J].

COLLIER, RH ;

KOHLHAW, G .

JOURNAL OF BACTERIOLOGY, 1972, 112 (01) :365-&

[5] ASSAY FOR TRANSAMINASE-B ENZYME-ACTIVITY IN ESCHERICHIA-COLI K-12 [J].

DUGGAN, DE ;

WECHSLER, JA .

ANALYTICAL BIOCHEMISTRY, 1973, 51 (01) :67-79

[6] ESCHERICHIA-COLI-K-12 MUTANT WITH ALTERNATE REQUIREMENTS FOR VITAMIN-B6 OR BRANCHED-CHAIN AMINO-ACIDS AND LACKING TRANSAMINASE C ACTIVITY [J].

FALKINHAM, JO .

JOURNAL OF BACTERIOLOGY, 1977, 130 (01) :566-568

[7] EXPRESSION OF A VALINE-RESISTANT ACETOLACTATE SYNTHASE ACTIVITY MEDIATED BY ILV-O AND ILV-G GENES OF ESCHERICHIA-COLI K-12 [J].

FAVRE, R ;

WIATER, A ;

PUPPO, S ;

IACCARINO, M ;

NOELLE, R ;

FREUNDLICH, M .

MOLECULAR AND GENERAL GENETICS, 1976, 143 (03) :243-252

[8] CONTROL OF ISOLEUCINE, VALINE, AND LEUCINE BIOSYNTHESIS .1. MULT-VALENT REPRESSION [J].

FREUNDLICH, M ;

BURNS, RO ;

UMBARGER, HE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1962, 48 (10) :1804-&

[9] ESCHERICHIA-COLI MUTANTS DEFICIENT IN ASPARTATE AND AROMATIC AMINO-ACID AMINOTRANSFERASES [J].

GELFAND, DH ;

STEINBERG, RA .

JOURNAL OF BACTERIOLOGY, 1977, 130 (01) :429-440

[10] *METHODE PERMETTANT LETUDE CONJUGUEE DES PROPRIETES ELECTROPHORETIQUES ET IMMUNOCHIMIQUES DUN MELANGE DE PROTEINES - APPLICATION AU SERUM SANGUIN [J].

GRABAR, P ;

WILLIAMS, CA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1953, 10 (01) :193-194