THE DISTRIBUTION OF CDNA SEQUENCES ON FIELD BEAN CHROMOSOMES

被引：6

作者：

HOUBEN, A ^{[1
]}

BRANDES, A ^{[1
]}

SCHUBERT, I ^{[1
]}

机构：

[1] INST PFLANZENGENET & KULTURPFLANZENFORSCH,D-06466 GATERSLEBEN,GERMANY

来源：

GENOME | 1994年 / 37卷 / 06期

关键词：

CDNA; FIELD BEAN; FLUORESCENT IN SITU HYBRIDIZATION;

D O I：

10.1139/g94-152

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Total polyadenylated RNA was isolated from different organs of the field bean by oligo-dT latex particles. Complementary DNA (cDNA) was reverse transcribed from the mRNAs and used as a probe for fluorescent in situ hybridization to metaphase chromosomes. Hybridization signals covered the whole chromosome complement except for late-replicating Giemsa-banded heterochromatin regions, which are composed mainly (or exclusively) of tandemly arranged repetitive sequences.

引用

页码：1065 / 1067

页数：3

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