SIMPLE IMMUNOFLUORESCENT TECHNIQUE FOR THE DETECTION OF HUMAN ROTAVIRUS

被引：15

作者：

MOOSAI, RB ^{[1
]}

GARDNER, PS ^{[1
]}

ALMEIDA, JD ^{[1
]}

GREENAWAY, MA ^{[1
]}

机构：

[1] WELLCOME RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF MEDICAL VIROLOGY | 1979年 / 3卷 / 03期

关键词：

enzyme action; immunofluorescence; rotavirus; trypsin;

D O I：

10.1002/jmv.1890030304

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

If trypsin is incorporated in the tissue culture medium it is possible to carry out a sensitive immunofluorescence assay for the presence of human rotavirus. The enhanced effect of trypsin is negated by serum. It has also been established that naturally occurring enzymes in faeces enable some virus to penetrate tissue culture cells. The role of these naturally occurring enzymes in the pathogenesis of rotavirus infection is discussed. Copyright © 1978 Wiley‐Liss, Inc., A Wiley Company

引用

页码：189 / 194

页数：6

共 14 条

[1] EFFECT OF TRYPSIN ON GROWTH OF ROTAVIRUS [J].

ALMEIDA, JD ;

HALL, T ;

BANATVALA, JE ;

TOTTERDELL, BM ;

CHRYSTIE, IL .

JOURNAL OF GENERAL VIROLOGY, 1978, 40 (JUL) :213-218

[2]

BABIUK LA, 1977, J CLIN MICROBIOL, V6, P610

[3]

BAKER SJ, 1966, INTRO MEDICAL LABORA, P249

[4]

BANATVALA JE, 1975, LANCET, P821

[5] STOOL TRYPSIN AND CHYMOTRYPSIN [J].

BARBERO, GJ ;

SIBINGA, MS ;

MARINO, JM ;

SEIBEL, R .

AMERICAN JOURNAL OF DISEASES OF CHILDREN, 1966, 112 (06) :536-&

[6]

BISHOP RF, 1973, LANCET, P1281

[7] CLASSIFICATION AND NOMENCLATURE OF VIRUSES - SUMMARY OF RESULTS OF MEETINGS OF INTERNATIONAL-COMMITTEE-ON-TAXONOMY-OF-VIRUSES IN MADRID, SEPTEMBER 1975 [J].

FENNER, F .

JOURNAL OF GENERAL VIROLOGY, 1976, 31 (JUN) :463-470

[8]

FLEWETT TH, 1973, LANCET, V2, P1497

[9]

GARDNER PS, 1974, RAPID VIRUS DIAGNOSI, P48

[10] RAPID DIAGNOSIS OF ROTAVIRUS INFECTIONS - COMPARISON OF ELECTRON-MICROSCOPY AND IMMUNOELECTRO-OSMOPHORESIS FOR DETECTION OF ROTAVIRUS IN HUMAN INFANTILE GASTROENTERITIS [J].

GRAUBALLE, PC ;

GENNER, J ;

MEYLING, A ;

HORNSLETH, A .

JOURNAL OF GENERAL VIROLOGY, 1977, 35 (MAY) :203-218

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