PHEROMONE RESPONSE IN YEAST - ASSOCIATION OF BEM1P WITH PROTEINS OF THE MAP KINASE CASCADE AND ACTIN

被引：181
作者：
LEEUW, T
FOURESTLIEUVIN, A
WU, CL
CHENEVERT, J
CLARK, K
WHITEWAY, M
THOMAS, DY
LEBERER, E
机构：
[1] NATL RES COUNCIL CANADA, BIOTECHNOL RES INST, EUKARYOT GENET GRP, MONTREAL, PQ H4P 2R2, CANADA
[2] UNIV CALIF SAN FRANCISCO, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA
来源：
SCIENCE | 1995年 / 270卷 / 5239期
关键词：
D O I：
10.1126/science.270.5239.1210
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Haploid cells of the yeast Saccharomyces cerevisiae respond to mating pheromones with polarized growth toward the mating partner. This morphological response requires the function of the cell polarity establishment protein Bem1p. Immunochemical and two-hybrid protein interaction assays revealed that Bem1p interacts with two components of the pheromone-responsive mitogen-activated protein (MAP) kinase cascade, Ste20p and Ste5p, as well as with actin. Mutants of Bem1p that are associated with defective pheromone-induced polarized morphogenesis interacted with Ste5p and actin but not with Ste20p. Thus, the association of Bem1p with Ste20p and Ste5p may contribute to the conveyance of spatial information that regulates polarized rearrangement of the actin cytoskeleton during yeast mating.
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页码：1210 / 1213
页数：4
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