FACILE SYNTHESIS OF L-MALIC ACID BY A CONSECUTIVE ENZYMATIC-REACTION

被引：0

作者：

CHEN, ST

DUH, SC

WANG, KT

机构：

[1] NATL TAIWAN UNIV,INST BIOCHEM SCI,TAIPEI,TAIWAN

[2] NATL TAIWAN UNIV,DEPT CHEM,TAIPEI 10764,TAIWAN

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Procedures for a consecutive reaction catalyzed by fumarase and followed by aspartase in ammonium fumarate buffer to facilitate the separation of product have been developed. Fumarate was converted to L-malate in 83%, and the rest of the starting material was converted to aspartate in 16.7% to make a total conversion of 99.7%. After the solvent was evaporated, the malic acid was dissolved in acetone, and the aspartic acid was insoluble. A simple filtration could separate both products.

引用

收藏

页码：355 / 358

页数：4

相关论文

共 12 条

[1] NEW PURIFICATION PROCEDURE FOR FUMARASE BASED OF AFFINITY CHROMATOGRAPHY - ISOLATION AND CHARACTERIZATION OF PIG-LIVER FUMARASE [J].

BEECKMANS, S ;

KANAREK, L .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 78 (02) :437-444

[2] STUDIES OF THE ENZYME FUMARASE .1. KINETICS AND EQUILIBRIUM [J].

BOCK, RM ;

ALBERTY, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1953, 75 (08) :1921-1925

[3] TEMPERATURE DEPENDENCE OF STEADY STATE KINETICPARAMETERS OF FUMARASE REACTION [J].

BRANT, DA ;

BARNETT, LB ;

ALBERTY, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (15) :2204-&

[4]

BRANT DA, 1963, J AM CHEM SOC, V47, P5709

[5] 2 ENANTIOSELECTIVE SYNTHESES OF A PRECURSOR OF THE BIOLOGICALLY MOST ACTIVE ISOMER OF CGA 80000 (CLOZYLACON) [J].

BUSER, HP ;

PUGIN, B ;

SPINDLER, F ;

SUTTER, M .

TETRAHEDRON, 1991, 47 (30) :5709-5716

[6] INDUSTRIAL SEPARATION OF CARBOXYLIC AND AMINO-ACIDS BY LIQUID MEMBRANES - APPLICABILITY, PROCESS CONSIDERATIONS, AND POTENTIAL ADVANTAGES [J].

EYAL, AM ;

BRESSLER, E .

BIOTECHNOLOGY AND BIOENGINEERING, 1993, 41 (03) :287-295

[7] STUDIES ON FUMARASE .4. THE EFFECTS OF INHIBITORS ON FUMARASE ACTIVITY [J].

MASSEY, V .

BIOCHEMICAL JOURNAL, 1953, 55 (01) :172-177

[8] D-MALIC ACID PRODUCTION FROM DL-MALIC ACID BY ENANTIOSPECIFIC ASSIMILATION WITH ACINETOBACTER-LWOFII [J].

MIYAMA, M ;

NAKAYAMA, K .

BIOTECHNOLOGY LETTERS, 1993, 15 (01) :23-28

[9] A STEREOSPECIFIC SYNTHESIS OF (-)-BESTATIN FROM L-MALIC ACID [J].

NORMAN, BH ;

MORRIS, ML .

TETRAHEDRON LETTERS, 1992, 33 (45) :6803-6806

[10] CHIRALITY AND CROP PROTECTION [J].

TOMBO, GMR ;

BELLUS, D .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 1991, 30 (10) :1193-1215