KIF1B, A NOVEL MICROTUBULE PLUS END-DIRECTED MONOMERIC MOTOR PROTEIN FOR TRANSPORT OF MITOCHONDRIA

被引：491

作者：

NANGAKU, M ^{[1
]}

SATOYOSHITAKE, R ^{[1
]}

OKADA, Y ^{[1
]}

NODA, Y ^{[1
]}

TAKEMURA, R ^{[1
]}

YAMAZAKI, H ^{[1
]}

HIROKAWA, N ^{[1
]}

机构：

[1] OKINAKA MEM INST MED RES, TOKYO 105, JAPAN

来源：

CELL | 1994年 / 79卷 / 07期

关键词：

D O I：

10.1016/0092-8674(94)90012-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To further elucidate the mechanism of organelle transport, we cloned a navel member of the mouse kinesin superfamily, KIF1B. This N-terminal-type motor protein is expressed ubiquitously in various kinds of tissues. In situ hybridization revealed that KIF1B is expressed abundantly in differentiated nerve cells. Interestingly, KIF1B works as a monomer, having a microtubule plus end-directed motility, Our rotary shadowing electron microscopy revealed mostly single globular structures. Immunocytochemically, KIF1B was colocalized with mitochondria in vivo. Furthermore, a subcellular fractionation study showed that KIF1B was concentrated in the mitochondrial fraction, and purified KIF1B could transport mitochondria along microtubules in vitro. These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria.

引用

页码：1209 / 1220

页数：12

共 72 条

[31] 2 SACCHAROMYCES-CEREVISIAE KINESIN-RELATED GENE-PRODUCTS REQUIRED FOR MITOTIC SPINDLE ASSEMBLY [J].