APPLICATION OF IMMOBILIZED AMINOACYLASE FROM MICROCOCCUS-AGILIS FOR ISOLATION OF D-PHENYLGLYCINE FROM ITS RACEMIC-MIXTURE

被引：5

作者：

SZWAJCER, E

SZEWCZUK, A

MORDARSKI, M

机构：

[1] Department of Microbiology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroctaw, Poland

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1981年 / 23卷 / 08期

关键词：

Aminoacylase - DEAE cellulose - Enzymatic reaction - L-enantiomers - L-phenylglycine - Phenylglycines - Racemic mixtures - Spectro-photometric method;

D O I：

10.1002/bit.260230802

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The procedure for isolation of D-phenylglycine from its racemic mixture by enzymatic hydrolysis of L-enantiomer of N-acetyl-D,L-phenylglycine is described. For this hydrolysis. aminoacylase from Micrococcus agilis immobilized by sorption of DEAE-cellulose was applied. As is also shown, the course of enzymatic reaction can be directly controlled by spectrophotometric method. © 1981 John Wiley & Sons, Inc.

引用

页码：1675 / 1681

页数：7

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