A FATTY-ACID-BINDING PROTEIN FROM WHEAT KERNELS

被引：11

作者：

CASTAGNARO, A ^{[1
]}

GARCIAOLMEDO, F ^{[1
]}

机构：

[1] UNIV POLITECN MADRID,ETS INGENIEROS AGRONOMOS,BIOQUIM & BIOL MOLEC LAB,E-28040 MADRID,SPAIN

来源：

FEBS LETTERS | 1994年 / 349卷 / 01期

关键词：

CEREAL; FATTY ACID BINDING PROTEIN; PLANT; WHEAT;

D O I：

10.1016/0014-5793(94)00660-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein of about 7 kDa (W-FABP) has been isolated from mature wheat kernels by H2O extraction and gel filtration of the extract, followed by two steps of high-performance liquid chromatography. The N-terminal amino acid sequence has been determined up to the 28th residue and found to be identical (except for positions 4 and 5) to that deduced from a barley cDNA (EMBL X15257), which had been improperly classified as a non-specific lipid transfer protein (LTP2). Similarly with LTPs, W-FABP does bind fatty acids, but in contrast, it is not significantly homologous to LTPs, it is not recognized by LTP antibodies, it has a more acidic isoelectric point (pH < 6.8 vs. pH > 9.6), and it does not show antibiotic properties.

引用

页码：117 / 119

页数：3

共 17 条

[11]

OLSEN OA, 1991, MAY P EUCARPIA S GEN

[12] ACYL-BINDING LIPID-TRANSFER PROTEINS FROM RAPE SEEDLINGS, A NOVEL CATEGORY OF PROTEINS INTERACTING WITH LIPIDS [J].

OSTERGAARD, J ;

VERGNOLLE, C ;

SCHOENTGEN, F ;

KADER, JC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1170 (02) :109-117

[13] A PHOSPHOLIPID TRANSFER PROTEIN THAT BINDS LONG-CHAIN FATTY-ACIDS [J].

RICKERS, J ;

SPENER, F ;

KADER, JC .

FEBS LETTERS, 1985, 180 (01) :29-32

[14] FATTY-ACID BINDING-PROTEINS .2. PURIFICATION AND BINDING CHARACTERISTICS OF A BASIC FATTY-ACID BINDING-PROTEIN FROM AVENA-SATIVA SEEDLINGS [J].

RICKERS, J ;

TOBER, I ;

SPENER, F .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 794 (02) :313-319

[15] PURIFICATION AND ANTIPATHOGENIC ACTIVITY OF LIPID TRANSFER PROTEINS (LTPS) FROM THE LEAVES OF ARABIDOPSIS AND SPINACH [J].

SEGURA, A ;

MORENO, M ;

GARCIAOLMEDO, F .

FEBS LETTERS, 1993, 332 (03) :243-246

[16] STRUCTURE AND EXPRESSION OF THE BARLEY LIPID TRANSFER PROTEIN GENE LTP1 [J].

SKRIVER, K ;

LEAH, R ;

MULLERURI, F ;

OLSEN, FL ;

MUNDY, J .

PLANT MOLECULAR BIOLOGY, 1992, 18 (03) :585-589

[17]

TCHANG F, 1988, J BIOL CHEM, V263, P16849

← 1 2 →