A FATTY-ACID-BINDING PROTEIN FROM WHEAT KERNELS

被引：11

作者：

CASTAGNARO, A ^{[1
]}

GARCIAOLMEDO, F ^{[1
]}

机构：

[1] UNIV POLITECN MADRID,ETS INGENIEROS AGRONOMOS,BIOQUIM & BIOL MOLEC LAB,E-28040 MADRID,SPAIN

来源：

FEBS LETTERS | 1994年 / 349卷 / 01期

关键词：

CEREAL; FATTY ACID BINDING PROTEIN; PLANT; WHEAT;

D O I：

10.1016/0014-5793(94)00660-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein of about 7 kDa (W-FABP) has been isolated from mature wheat kernels by H2O extraction and gel filtration of the extract, followed by two steps of high-performance liquid chromatography. The N-terminal amino acid sequence has been determined up to the 28th residue and found to be identical (except for positions 4 and 5) to that deduced from a barley cDNA (EMBL X15257), which had been improperly classified as a non-specific lipid transfer protein (LTP2). Similarly with LTPs, W-FABP does bind fatty acids, but in contrast, it is not significantly homologous to LTPs, it is not recognized by LTP antibodies, it has a more acidic isoelectric point (pH < 6.8 vs. pH > 9.6), and it does not show antibiotic properties.

引用

页码：117 / 119

页数：3

共 17 条

[1] TRANSCRIPTS ENCODING AN OLEOSIN AND A DORMANCY-RELATED PROTEIN ARE PRESENT IN BOTH THE ALEURONE LAYER AND THE EMBRYO OF DEVELOPING BARLEY (HORDEUM-VULGARE L) SEEDS
AALEN, RB
OPSAHLFERSTAD, HG
LINNESTAD, C
OLSEN, OA
[J]. PLANT JOURNAL, 1994, 5 (03) : 385 - 396
[2] AMINO-ACID-SEQUENCE OF A NONSPECIFIC WHEAT PHOSPHOLIPID TRANSFER PROTEIN AND ITS CONFORMATION AS REVEALED BY INFRARED AND RAMAN-SPECTROSCOPY - ROLE OF DISULFIDE BRIDGES AND PHOSPHOLIPIDS IN THE STABILIZATION OF THE ALPHA-HELIX STRUCTURE
DESORMEAUX, A
BLOCHET, JE
PEZOLET, M
MARION, D
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1121 (1-2) : 137 - 152
[3] NUCLEOTIDE-SEQUENCE OF A CDNA-ENCODING A LIPID TRANSFER PROTEIN FROM WHEAT (TRITICUM-DURUM DESF)
DIERYCK, W
GAUTIER, MF
LULLIEN, V
JOUDRIER, P
[J]. PLANT MOLECULAR BIOLOGY, 1992, 19 (04) : 707 - 709
[4] DOOLITTLE RF, 1986, URFS ORFS, P1
[5] CLONING OF CDNA AND CHROMOSOMAL LOCATION OF GENES ENCODING THE 3 TYPES OF SUBUNITS OF THE WHEAT TETRAMERIC INHIBITOR OF INSECT ALPHA-AMYLASE
GARCIAMAROTO, F
MARANA, C
MENA, M
GARCIAOLMEDO, F
CARBONERO, P
[J]. PLANT MOLECULAR BIOLOGY, 1990, 14 (05) : 845 - 853
[6] BARLEY ALEURONE CELL-DEVELOPMENT - MOLECULAR-CLONING OF ALEURONE-SPECIFIC CDNAS FROM IMMATURE GRAINS
JAKOBSEN, K
KLEMSDAL, SS
AALEN, RB
BOSNES, M
ALEXANDER, D
OLSEN, OA
[J]. PLANT MOLECULAR BIOLOGY, 1989, 12 (03) : 285 - 293
[7] PROMOTER OF A LIPID TRANSFER PROTEIN GENE EXPRESSED IN BARLEY ALEURONE CELLS CONTAINS SIMILAR MYB AND MYC RECOGNITION SITES AS THE MAIZE BZ-MCC ALLELE
LINNESTAD, C
LONNEBORG, A
KALLA, R
OLSEN, OA
[J]. PLANT PHYSIOLOGY, 1991, 97 (02) : 841 - 843
[8] DEVELOPMENTAL AND PATHOGEN-INDUCED EXPRESSION OF 3 BARLEY GENES ENCODING LIPID TRANSFER PROTEINS
MOLINA, A
GARCIAOLMEDO, F
[J]. PLANT JOURNAL, 1993, 4 (06) : 983 - 991
[9] LIPID TRANSFER PROTEINS (NSLTPS) FROM BARLEY AND MAIZE LEAVES ARE POTENT INHIBITORS OF BACTERIAL AND FUNGAL PLANT-PATHOGENS
MOLINA, A
SEGURA, A
GARCIAOLMEDO, F
[J]. FEBS LETTERS, 1993, 316 (02) : 119 - 122
[10] OLSEN OA, 1990, PLANTA, V181, P462, DOI 10.1007/BF00192998

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