Longidorus silvae sp. n. is described and illustrated. It was found in natural habitat, woodland, at Ampezzo, province of Udine and at Palazzetto, province of Siena, Italy. The new species is similar to L. goodeyi Hooper, 1961, L. major Roca & D'Errico, 1987 and L. picenus Roca, Lamberti & Agostinelli, 1985. L. silvae sp. n. is characterized by the lip region continuous with the rest of the body, unevenly bilobed amphidial pouches; the female tail is short and rounded and the first larval stage has a long tail mucro.