THE RAT BETA-3-ADRENERGIC RECEPTOR GENE CONTAINS AN INTRON

被引:41
作者
BENSAID, M [1 ]
KAGHAD, M [1 ]
RODRIGUEZ, M [1 ]
LEFUR, G [1 ]
CAPUT, D [1 ]
机构
[1] SANOFI ELF BIORECH,BIOL MOLEC LAB,BP 137,F-31676 LABEGE,FRANCE
关键词
BETA-3-ADRENERGIC RECEPTOR; INTRON; POLYMERASE CHAIN REACTION;
D O I
10.1016/0014-5793(93)80516-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report that the rat beta3-adrenergic receptor (beta3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta3-AR were both deduced from genomic DNA sequences. Given the heterogeneity at the C-termini of the otherwise highly similar rat, mouse and human sequences, we discuss the intriguing possibility that the beta3-AR gene of the latter two species also contain an intron near the extremity of the open reading frame. A beta-adrenergic receptor (beta-AR) cDNA we have cloned from rat colonic tissue which has a sequence essentially identical to that previously reported for the rat adipose beta3-AR cDNA [(1991) J. Chem. 266, 24053], encodes the spliced version of the beta3-AR.
引用
收藏
页码:223 / 226
页数:4
相关论文
共 24 条
[1]   ATYPICAL BETA-ADRENOCEPTOR ON BROWN ADIPOCYTES AS TARGET FOR ANTI-OBESITY DRUGS [J].
ARCH, JRS ;
AINSWORTH, AT ;
CAWTHORNE, MA ;
PIERCY, V ;
SENNITT, MV ;
THODY, VE ;
WILSON, C ;
WILSON, S .
NATURE, 1984, 309 (5964) :163-165
[3]   INVITRO INHIBITION OF INTESTINAL MOTILITY BY PHENYLETHANOLAMINOTETRALINES - EVIDENCE OF ATYPICAL BETA-ADRENOCEPTORS IN RAT COLON [J].
BIANCHETTI, A ;
MANARA, L .
BRITISH JOURNAL OF PHARMACOLOGY, 1990, 100 (04) :831-839
[4]   IDENTIFICATION OF A COMMON NUCLEOTIDE-SEQUENCE IN THE 3'-UNTRANSLATED REGION OF MESSENGER-RNA MOLECULES SPECIFYING INFLAMMATORY MEDIATORS [J].
CAPUT, D ;
BEUTLER, B ;
HARTOG, K ;
THAYER, R ;
BROWNSHIMER, S ;
CERAMI, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (06) :1670-1674
[5]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[6]   THE DOPAMINE-D2 RECEPTOR - 2 MOLECULAR-FORMS GENERATED BY ALTERNATIVE SPLICING [J].
DALTOSO, R ;
SOMMER, B ;
EWERT, M ;
HERB, A ;
PRITCHETT, DB ;
BACH, A ;
SHIVERS, BD ;
SEEBURG, PH .
EMBO JOURNAL, 1989, 8 (13) :4025-4034
[7]   MODEL SYSTEMS FOR THE STUDY OF 7-TRANSMEMBRANE-SEGMENT RECEPTORS [J].
DOHLMAN, HG ;
THORNER, J ;
CARON, MG ;
LEFKOWITZ, RJ .
ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :653-688
[8]   MOLECULAR CHARACTERIZATION OF THE HUMAN BETA-3-ADRENERGIC RECEPTOR [J].
EMORINE, LJ ;
MARULLO, S ;
BRIENDSUTREN, MM ;
PATEY, G ;
TATE, K ;
DELAVIERKLUTCHKO, C ;
STROSBERG, AD .
SCIENCE, 1989, 245 (4922) :1118-1121
[9]  
EMORINE LJ, 1991, ADV PHAR SC, P79
[10]   COLONY HYBRIDIZATION - METHOD FOR ISOLATION OF CLONED DNAS THAT CONTAIN A SPECIFIC GENE [J].
GRUNSTEIN, M ;
HOGNESS, DS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) :3961-3965