IN-VITRO FUNCTIONS OF THE RAT DIAPHRAGM DURING POSTNATAL-DEVELOPMENT

This study was designed to determine the developmental changes in the functional characteristics of the rat diaphragm. A total of 150 animals were studied at 1, 3, 6, 7 and 9 weeks of postnatal age. Body and diaphragm muscle weights were measured. Diaphragm strips were studied in an in vitro preparation to assess muscle contractile and endurance properties. Total diaphragm weight increased considerably, by a factor of 23 over the 9 week-period of study and was highly correlated with body weight (r=0.93, P<0.01). However, the ratio of diaphragm-to-body weight decreased progressively with age. In comparison with those from older animals, diaphragms from 1 and 3 weeks old animals: (1) generated similar force normalized for muscle weight but a lower force normalized for fibre cross-sectional area (P<0.05), (2) had longer time-to-peak tension and one-half relaxation times (P<0.01) and (3) were more resistant to fatigue (P<0.01). The mechanisms underlying the diaphragm functional development were discussed.