EFFECT OF POTATO GLYCOALKALOIDS, ALPHA-CHACONINE AND ALPHA-SOLANINE, ON MEMBRANE-POTENTIAL OF FROG EMBRYOS

被引：50

作者：

BLANKEMEYER, JT ^{[1
]}

STRINGER, BK ^{[1
]}

RAYBURN, JR ^{[1
]}

BANTLE, JA ^{[1
]}

FRIEDMAN, M ^{[1
]}

机构：

[1] USDA ARS, W REG RES CTR, FOOD SAFETY RES UNIT, ALBANY, CA 94710 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1992年 / 40卷 / 10期

关键词：

D O I：

10.1021/jf00022a057

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

To demonstrate whether potato glycoalkaloids can alter the integrity of membranes of frog embryo, albino frog embryos were incubated with alpha-chaconine and alpha-solanine. Di-4-ANEPPS, an electrochromic fluorescent dye, was added to measure embryonic membrane potential. Alpha-Chaconine increased the Di-4-ANEPPS fluorescence up to 1600% of control, alpha-solanine increased the fluorescence up to 400%, and solanidine had no effect. Increases in fluorescence, when plotted in a concentration-response format, produced EC50 values near published values for FETAX (frog embryo teratogenicity assay-Xenopus). Possible mechanisms and the significance of the fluorescence results to food safety are discussed.

引用

页码：2022 / 2025

页数：4

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