SEPARATION OF TAXOL FROM RELATED TAXANES IN TAXUS-BREVIFOLIA EXTRACTS BY ISOCRATIC ELUTION REVERSED-PHASE MICROCOLUMN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：31

作者：

HARVEY, SD ^{[1
]}

CAMPBELL, JA ^{[1
]}

KELSEY, RG ^{[1
]}

VANCE, NC ^{[1
]}

机构：

[1] USDA, PACIFIC NW RES STN, CORVALLIS, OR 97331 USA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 587卷 / 02期

关键词：

D O I：

10.1016/0021-9673(91)85172-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Previous attempts to resolve the closely related taxanes cephalomannine and taxol by reversed-phase high-performance liquid chromatography (HPLC) on octadecyl silica columns have not been successful. In the present study, high-resolution HPLC on microcolumns packed with octadecyl silica was evaluated for the separation of the aforementioned taxanes. Due to the enhanced separation efficiency of this technique, baseline resolution between cephalomannine and taxol was readily achieved under isocratic elution conditions. Chromatographic profiles of Taxus brevifolia twig and needle foliage extracts are presented that illustrate this separation.

引用

页码：300 / 305

页数：6

共 25 条

[1] HIGH-EFFICIENCY SEPARATION AND CHARACTERIZATION OF HIGH-MOLECULAR-WEIGHT HETEROCYCLIC SULFUR-COMPOUNDS IN FOSSIL-FUELS BY MICROCOLUMN LIQUID-CHROMATOGRAPHY AND RELATED TECHNIQUES [J].