PURIFICATION AND PARTIAL CHARACTERIZATION OF RAT-LIVER SOLUBLE CATECHOL-O-METHYLTRANSFERASE

被引：37
作者：
TILGMANN, C
KALKKINEN, N
机构：
[1] UNIV HELSINKI,INST BIOTECHNOL,KARVAAMOKUJA 3,SF-00380 HELSINKI,FINLAND
[2] ORION CORP,GENET ENGN LAB,SF-00380 HELSINKI,FINLAND
来源：
FEBS LETTERS | 1990年 / 264卷 / 01期
关键词：
Catechol-O-methyltransferase; soluble; Methylating enzyme;
D O I：
10.1016/0014-5793(90)80774-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The rat liver soluble catechol-O-methyltransferase (EC 2.1.1.6.) has been purified utilizing a combination of conventional chromatography and HPLC. The purified enzyme has a molecular mass of 25 kDa, a pI of 5.1, and exists in two forms which differ in the nature of their intramolecular disulfide bonds. This difference causes these two protein forms to behave differently in reversed phase chromatography. © 1990.
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页码：95 / 99
页数：5
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