TERMINAL DEOXYNUCLEOTIDYLTRANSFERASE CONTAINING MEGADALTON COMPLEX FROM YOUNG-RAT THYMUS NUCLEI - IDENTIFICATION AND CHARACTERIZATION

被引：11

作者：

PANDEY, VN

DAVE, VP

PATIL, MS

PRADHAN, DS

AMRUTE, SB

MODAK, MJ

机构：

[1] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT BIOCHEM & MOLEC BIOL,NEWARK,NJ 07103

[2] BHABHA ATOM RES CTR,DIV BIOCHEM,BOMBAY 400085,INDIA

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 17期

关键词：

D O I：

10.1021/bi00469a002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear matrix prepared from 2–3 week old rat thymuses contains tightly bound TdT activity which has been quantitatively solubilized with nonionic detergent and sonication. TdT is contained in a discrete complex with a sedimentation value of 23 S. The complex is retained on an anti-TdT antibody column and contains DNA ligase and 3′-5′ exonuclease activities as well as DNA and several other proteins but is devoid of replicative DNA polymerases. Such a type of multienzyme complex is absent from the nuclear extracts of thymus prepared from older rats and also from liver and spleen extracts of young and old rats. © 1990, American Chemical Society. All rights reserved.

引用

页码：4037 / 4041

页数：5

共 24 条

[21] NUCLEAR MATRIX-BOUND DEOXYRIBONUCLEIC-ACID SYNTHESIS - AN INVITRO SYSTEM [J].