TERMINAL DEOXYNUCLEOTIDYLTRANSFERASE CONTAINING MEGADALTON COMPLEX FROM YOUNG-RAT THYMUS NUCLEI - IDENTIFICATION AND CHARACTERIZATION

被引：11
作者：
PANDEY, VN
DAVE, VP
PATIL, MS
PRADHAN, DS
AMRUTE, SB
MODAK, MJ
机构：
[1] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT BIOCHEM & MOLEC BIOL,NEWARK,NJ 07103
[2] BHABHA ATOM RES CTR,DIV BIOCHEM,BOMBAY 400085,INDIA
来源：
BIOCHEMISTRY | 1990年 / 29卷 / 17期
关键词：
D O I：
10.1021/bi00469a002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nuclear matrix prepared from 2–3 week old rat thymuses contains tightly bound TdT activity which has been quantitatively solubilized with nonionic detergent and sonication. TdT is contained in a discrete complex with a sedimentation value of 23 S. The complex is retained on an anti-TdT antibody column and contains DNA ligase and 3′-5′ exonuclease activities as well as DNA and several other proteins but is devoid of replicative DNA polymerases. Such a type of multienzyme complex is absent from the nuclear extracts of thymus prepared from older rats and also from liver and spleen extracts of young and old rats. © 1990, American Chemical Society. All rights reserved.
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页码：4037 / 4041
页数：5
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