DNA COMPARISONS OF TRYPANOSOMA-EVANSI (INDONESIA) AND TRYPANOSOMA-BRUCEI SPP

被引：73

作者：

ARTAMA, WT ^{[1
]}

AGEY, MW ^{[1
]}

DONELSON, JE ^{[1
]}

机构：

[1] UNIV IOWA,DEPT BIOCHEM,HOWARD HUGHES MED INST,IOWA CITY,IA 52242

来源：

PARASITOLOGY | 1992年 / 104卷

关键词：

TRYPANOSOMA-EVANSI; MINICIRCLE DNA; POLYMERASE CHAIN REACTION; GENE REPEATS;

D O I：

10.1017/S0031182000060819

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Two clones from separate isolates of Trypanosoma evansi in Indonesia were found by polymerase chain reaction (PCR) analyses to contain 3 different repeated nuclear DNA sequences of Trypanosoma brucei spp: the consensus sequence for a highly repetitive 177 base pairs and the gene repeats encoding procyclin and the spliced leader. In addition, the 994 bp minicircle sequence of one of the clones was determined, and PCR amplification primers specific for minicircles of T. evansi were identified that do not amplify minicircle sequences in the T. brucei spp. clones tested.

引用

页码：67 / 74

页数：8

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