STATE OF TYROSINES OF BOVINE PANCREATIC RIBONUCLEASE IN ETHYLENE GLYCOL AND GLYCEROL

被引：23

作者：

BELLO, J

机构：

[1] Department of Biophysics, Roswell Park Memorial Institute, New York 14203, Buffalo

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 11期

关键词：

D O I：

10.1021/bi00839a046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The state of the tyrosines of bovine pancreatic ribonuclease was investigated in aqueous ethylene glycol and glycerol. By spectrophotometry, circular dichroism, and optical rotation, a conformational transition was found at all concentrations of polyol up to and including 97 %. Up to 75% glycol or glycerol, the thermal melting profile by optical rotation is sigmoid, with [a] becoming more negative with rising temperature. At 75% glycol or glycerol no transition is seen; but above 75% the melting profile is bell shaped, with [α] becoming first more positive and then more negative. Light scattering measurements give similar bell-shaped profiles, showing increased and then decreased scattering. The value of Tm extrapolated to neat glycol is close to 25°. All three optical methods show little change in conformation up to 90% glycol or glycerol. Marked changes occur between 90 and 97% polyol. This is confirmed by nitration in glycol, but for glycerol there is little nitration even at 93% glycerol. © 1969, American Chemical Society. All rights reserved.

引用

页码：4535 / &

共 8 条

[1]

BIGELOW CC, 1961, J BIOL CHEM, V236, P1706

[2]

HERSKOVITS TT, 1968, J BIOL CHEM, V243, P2123

[3] INTERMOLECULAR CROSS LINKING OF PROTEIN IN CRYSTALLINE STATE - CARBOXYPEPTIDASE-A [J].