CLONING AND TISSUE DISTRIBUTION OF THE HUMAN 11-BETA-HYDROXYSTEROID DEHYDROGENASE TYPE-2 ENZYME

被引:692
作者
ALBISTON, AL [1 ]
OBEYESEKERE, VR [1 ]
SMITH, RE [1 ]
KROZOWSKI, ZS [1 ]
机构
[1] BAKER INST MED RES,MELBOURNE,VIC 3181,AUSTRALIA
关键词
METABOLISM; KIDNEY; PLACENTA; PANCREAS; TESTIS; EXPRESSION CLONING;
D O I
10.1016/0303-7207(94)90176-7
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) is thought to protect the non-selective mineralocorticoid receptor from occupation by glucocorticoids, and to modulate access of glucocorticoids to glucocorticoid receptors resulting in protection of the fetus and gonads. A ubiquitous low affinity NADP(+) dependent enzyme (11 beta HSD1) and a tissue specific, high affinity NAD(+) dependent form (11 beta HSD2) of 11 beta HSD exist. We now report the isolation of a cDNA coding for human 11 beta HSD2. The new isoform is NAD(+) dependent, exclusively dehydrogenase in directionality, inhibited by glycyrrhetinic acid and metabolizes the synthetic glucocorticoid dexamethasone; it displays K-m values for corticosterone and cortisol of 5.1 nM and 47 nM, respectively. Sequence alignment shows that 11 beta HSD2 shares 35% identity with 17 beta HSD2, but is only 14% identical with 11 beta HSD1. The 11 beta HSD2 gene is highly expressed in kidney, colon, pancreas and placenta and the message is also present in the ovary, prostate and testis. These data suggest that 11 beta HSD2 plays an important role in modulating mineralocorticoid and glucocorticoid receptor occupancy by glucocorticoids.
引用
收藏
页码:R11 / R17
页数:7
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