GENETIC-RELATIONSHIPS DETERMINED BY A DNA HETERODUPLEX MOBILITY ASSAY - ANALYSIS OF HIV-1 ENV GENES

被引：731

作者：

DELWART, EL

SHPAER, EG

LOUWAGIE, J

MCCUTCHAN, FE

GREZ, M

RUBSAMENWAIGMANN, H

MULLINS, JI

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT MICROBIOL & IMMUNOL,STANFORD,CA 94305

[2] FDN ADVANCEMENT MIL MED,ROCKVILLE,MD 20850

[3] GEORG SPEYER HAUS CHEMOTHERAPEUT FORSCHUNGSINST,D-700251 FRANKFURT,GERMANY

来源：

SCIENCE | 1993年 / 262卷 / 5137期

关键词：

D O I：

10.1126/science.8235655

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The genetic diversity of human immunodeficiency virus (HIV) is a major concern thought to impact on immunologic escape and eventual vaccine efficacy. Here, simple and rapid methods are described for the detection and estimation of genetic divergence between HIV strains on the basis of the observation that DNA heteroduplexes formed between related sequences have a reduced mobility in polyacrylamide gels proportional to their degree of divergence. Reliable phylogenetic subtypes were assigned for HIV-1 strains from around the world. Relationships between viruses were closest when derived from the same or epidemiologically linked individuals. When derived from epidemiologically unlinked individuals, the relationships between viruses in a given geographic region correlated with the length of time HIV-1 had been detected in the population and the number of strains initiating widespread infection. Heteroduplex mobility analysis thus provides a tool to expedite epidemiological investigations by assisting in the classification of HIV and is readily applicable to the screening and characterization of other infectious agents and cellular genes.

引用

页码：1257 / 1261

页数：5

共 46 条

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