TRANSFORMATION OF GLUCONOBACTER-OXYDANS SUBSP SUBOXYDANS BY ELECTROPORATION

被引：7

作者：

CREAVEN, M ^{[1
]}

FITZGERALD, RJ ^{[1
]}

OGARA, F ^{[1
]}

机构：

[1] NATL UNIV IRELAND UNIV COLL CORK,DEPT MICROBIOL,CORK,IRELAND

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1994年 / 40卷 / 06期

关键词：

GLUCONOBACTER SUBOXYDANS; ELECTROPORATION; FIELD STRENGTH;

D O I：

10.1139/m94-079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An efficient electroporation system for Gluconobacter oxydans subsp. suboxydans was developed that gave transformation frequencies of up to 10(5) transformants/mu g of DNA. The system was studied with respect to different operating parameters including (i) the effect of electrical conditions, i.e., field strength and resistance, (ii) DNA concentration, (iii) composition of the electroporation buffer, (iv) initial cell concentration, and (v) stage of growth of the cells. Optimal electroporation conditions were found under the following conditions: 12.5 kV/cm field strength, 400 Omega parallel resistor setting, 0.4-0.5 mu g DNA/100 mu L cell suspension, and electroporation buffer containing ME ions and >10(11) colony-forming units/mL of early to mid log phase cells.

引用

页码：491 / 494

页数：4

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