HIGH EXPRESSION OF A NOVEL CARNITINE PALMITOYLTRANSFERASE-I LIKE PROTEIN IN RAT BROWN ADIPOSE-TISSUE AND HEART - ISOLATION AND CHARACTERIZATION OF ITS CDNA CLONE

被引：114

作者：

YAMAZAKI, N ^{[1
]}

SHINOHARA, Y ^{[1
]}

SHIMA, A ^{[1
]}

TERADA, H ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,FAC PHARMACEUT SCI,TOKUSHIMA 770,JAPAN

来源：

FEBS LETTERS | 1995年 / 363卷 / 1-2期

关键词：

BROWN ADIPOSE TISSUE; DIFFERENTIAL SCREENING; CDNA CLONING; MITOCHONDRION; CARNITINE PALMITOYLTRANSFERASE; RAT;

D O I：

10.1016/0014-5793(95)00277-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart.

引用

页码：41 / 45

页数：5