Chronic Suppurative Otitis Media (CSOM) is a condition of the middle ear that is characterized by persistent or recurrent discharge through a chronic perforation of the tympanic membrane from the middle ear cleft. Untreated cases of Chronic Suppurative Otitis Media can result in a broad range of complications like mastoiditis, labyrinthitis, facial nerve paralysis and serious intracranial complications. In this study an attempt is made to know the aerobic bacteriology of Chronic Suppurative Otitis Media, with antimicrobial susceptibility testing of the bacterial isolates. The present prospective study was carried out on 50 patients presenting with Chronic Suppurative Otitis Media between January 2014 to December 2014 in the department of E.N.T, Basaveshwara Medical College Hospital and Research Centre, Chitradurga. The most common bacteria in Chronic Suppurative Otitis Media in this study was Pseudomonas aeruginosa (62%) followed by Staphylococcus aureus (22%). Gentamicin was the most sensitive (100%) antibiotic against Pseudomonas aeruginosa followed by Amikacin (96.77%). Ceftriaxone and Gentamicin were the most sensitive (100%) antibiotic against Staphylococcus aureus followed by Amikacin (90.9%)