NADPH-CYTOCHROME-C (P-450) REDUCTASE HAS THE ACTIVITY OF NADPH-LINKED AQUACOBALAMIN REDUCTASE IN RAT-LIVER MICROSOMES

被引：12
|
作者：
WATANABE, F [1 ]
NAKANO, Y [1 ]
SAIDO, H [1 ]
TAMURA, Y [1 ]
YAMANAKA, H [1 ]
机构：
[1] UNIV OSAKA PREFECTURE,DEPT AGR CHEM,SAKAI,OSAKA 591,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1119卷 / 02期
关键词：
VITAMIN-B12; COBALAMIN; AQUACOBALAMIN REDUCTASE; NADPH-CYTOCHROME-C (P-450) REDUCTASE; (RAT LIVER MICROSOME);
D O I：
10.1016/0167-4838(92)90388-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To elucidate the mammalian system for synthesis of cobalamin coenzymes, microsomal NADPH-linked aquacobalamin reductase was purified and characterized. The enzyme was purified about 534-fold over rat liver microsomal fraction in a yield of about 32%. The purified enzyme was homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and had a monomeric molecular weight of 79000. The purified aquacobalamin reductase showed a high specific activity (about 55-mu-mol/min per mg protein) of NADPH-cytochrome c (P-450) reductase. About 33% of the NADPH-cytochrome c reductase activity found in the microsomal fraction was recovered in the final purified preparation. The activity ratio of NADPH-cytochrome c reductase/NADPH-linked aquacobalamin reductase was about 5.0 through the purification steps, indicating that the rat liver microsomal NADPH-linked aquacobalamin reductase is the NADPH-cytochrome c reductase.
引用
收藏
页码：175 / 177
页数：3
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