MINIATURIZATION OF 3 CARBOHYDRATE ANALYSES USING A MICROSAMPLE PLATE READER

被引：359

作者：

FOX, JD ^{[1
]}

ROBYT, JF ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT BIOCHEM & BIOPHYS,AMES,IA 50011

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90300-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and d-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by α-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated. © 1991.

引用

页码：93 / 96

页数：4

共 10 条

[1] USE OF A 96-WELL MICROPLATE READER FOR MEASURING ROUTINE ENZYME-ACTIVITIES [J].

ASHOUR, MBA ;

GEE, SJ ;

HAMMOCK, BD .

ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) :353-360

[2] A SEMIAUTOMATED SYSTEM FOR MEASUREMENT OF 96 SIMULTANEOUS SPECTROPHOTOMETRIC ENZYME ASSAYS [J].

BUTLER, EG ;

GILL, R ;

GARYCH, L .

ANALYTICAL BIOCHEMISTRY, 1988, 170 (02) :402-408

[3] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[4] DETERMINATION OF GLUCOSE IN PRESENCE OF MALTOSE AND ISOMALTOSE BY A STABLE, SPECIFIC ENZYMIC REAGENT [J].

FLEMING, ID ;

PEGLER, HF .

ANALYST, 1963, 88 (105) :967-&

[5] ADAPTATION OF THE NELSON-SOMOGYI REDUCING-SUGAR ASSAY TO A MICROASSAY USING MICROTITER PLATES [J].

GREEN, F ;

CLAUSEN, CA ;

HIGHLEY, TL .

ANALYTICAL BIOCHEMISTRY, 1989, 182 (02) :197-199

[6] STRUCTURE STUDIES OF AMYLOSE-V COMPLEXES AND RETROGRADED AMYLOSE BY ACTION OF ALPHA-AMYLASES, AND A NEW METHOD FOR PREPARING AMYLODEXTRINS [J].

JANE, JL ;

ROBYT, JF .

CARBOHYDRATE RESEARCH, 1984, 132 (01) :105-118

[7] COLORIMETRIC DETERMINATION OF NEUTRAL SUGARS BY A RESORCINOL SULFURIC-ACID MICROMETHOD [J].

MONSIGNY, M ;

PETIT, C ;

ROCHE, AC .

ANALYTICAL BIOCHEMISTRY, 1988, 175 (02) :525-530

[8] OXIDATION OF GLUCOSE + RELATED COMPOUNDS BY GLUCOSE OXIDASE FROM ASPERGILLUS NIGER [J].

PAZUR, JH ;

KLEPPE, K .

BIOCHEMISTRY, 1964, 3 (04) :578-&

[9]

Stein EA, 1961, BIOCHEM PREP, V8, P34

[10] ASSAY OF REDUCING SUGARS IN THE NANOMOLE RANGE WITH 2,2'-BICINCHONINATE [J].

WAFFENSCHMIDT, S ;

JAENICKE, L .

ANALYTICAL BIOCHEMISTRY, 1987, 165 (02) :337-340

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