PROSTAGLANDIN-D2 METABOLITE STIMULATES COLLAGEN-SYNTHESIS BY HUMAN OSTEOBLASTS DURING CALCIFICATION

被引：45

作者：

TASAKI, Y ^{[1
]}

TAKAMORI, R ^{[1
]}

KOSHIHARA, Y ^{[1
]}

机构：

[1] TOKYO METROPOLITAN GERIATR HOSP & INST GERONTOL,DEPT PHARMACOL,35-2 SAKAE CHO,ITABASHI KU,TOKYO 173,JAPAN

来源：

PROSTAGLANDINS | 1991年 / 41卷 / 04期

关键词：

D O I：

10.1016/0090-6980(91)90001-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the effect of the prostaglandin D2 metabolite DELTA-12-PGJ2 (9-Deoxy-DELTA-9, DELTA-12-13, 14-dihydroprostaglandin D2) on collagen synthesis in human osteoblasts. DELTA-12-PGJ2 at 10(-5)M enhanced collagen synthesis in the presence of 2 mM alpha-glycerophosphate-2Na. The stimulative effect appeared as early as 3 days after addition and continued until 22 days. The enhancement of type I collagen synthesis was confirmed by polyacrylamide gel electrophoresis. The potency was the same as 10(-8)M 1 alpha, 25 dihydroxy vitamin D3 (1,25 (OH)2D3). Northern blot analysis showed that 10(-5)M DELTA-12-PGJ2 and 10(-8)M 1,25 (OH) 2D3 enhanced the transcription of type I procollagen (alpha-1) mRNA levels in osteoblasts.

引用

页码：303 / 313

页数：11

共 19 条

[1] EFFECT OF PROSTAGLANDINS-E1 AND F1ALPHA ON BIOSYNTHESIS OF COLLAGEN [J].