DETERMINATION OF HEMIHEDRAL TWINNING AND INITIAL STRUCTURAL-ANALYSIS OF CRYSTALS OF THE PROCARBOXYPEPTIDASE-A TERNARY COMPLEX

被引：18

作者：

GOMISRUTH, FX

FITA, I

KIEFERSAUER, R

HUBER, R

AVILES, FX

NAVAZA, J

机构：

[1] ETS ENGN IND,DEPT ENGN QUIM,E-08028 BARCELONA,SPAIN

[2] UNIV AUTONOMA BARCELONA,INST BIOL FONAMENTAL,E-08193 BARCELONA,SPAIN

[3] UNIV AUTONOMA BARCELONA,DEPT BIOQUIM,E-08193 BARCELONA,SPAIN

[4] MAX PLANCK INST BIOCHEM,STRUKT FORSCH ABT,D-82152 MARTINSRIED,GERMANY

[5] CTR PHARMACEUT,PHYS LAB,CNRS,UPR180,F-92290 CHATENAY MALABRY,FRANCE

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1995年 / 51卷

关键词：

D O I：

10.1107/S0907444995000643

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The initial structural analysis of the ternary complex of procarboxypeptidase A from hemihedrally twinned crystals diffracting up to 2.8 Angstrom is described. Detection of twinning by different techniques is presented, including biochemical and intensity statistics approaches. The structure was initially solved using Patterson-search techniques, and the three positioned search models were used to effectively deconvolute the twinned data.

引用

页码：819 / 823

页数：5

共 18 条

[1]

AVILES FX, 1993, EUR J BIOCHEM, V166, P379

[2] DETERMINATION OF THE PROTEIN-CONTENT OF CRYSTALS FORMED BY MASTIGOCLADUS-LAMINOSUS C-PHYCOCYANIN, CHROOMONAS-SPEC PHYCOCYANIN-645 AND MODIFIED HUMAN-FIBRINOGEN USING AN IMPROVED FICOLL DENSITY GRADIENT-METHOD [J].