SELECTION OF MUTANTS OF ASPERGILLUS-NIGER SHOWING ENHANCED PRODUCTIVITY OF CITRIC-ACID FROM STARCH IN SHAKING CULTURE

被引：3

作者：

RUGSASEEL, S ^{[1
]}

KIRIMURA, K ^{[1
]}

USAMI, S ^{[1
]}

机构：

[1] WASEDA UNIV,SCH SCI & ENGN,DEPT APPL CHEM,TOKYO 160,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 75卷 / 03期

关键词：

D O I：

10.1016/0922-338X(93)90122-O

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mutants with enhanced citric acid production from soluble starch were induced from Aspergillus niger WU-2223L. After UV-irradiation of a conidial suspension of strain WU-2223L, mutants were selected on modified starch-methyl red agar plates on the basis of higher amylolytic activity and acid productivity. The 8 mutants selected showed enhanced citric acid production from soluble starch in shaking culture. Among them, a representative mutant strain, 2M-43, produced 48.0 g/l of citric acid from 120 g/l of soluble starch in 9 d of cultivation in shaking culture, whereas strain WU-2223L produced 35.1 g/l. Glucoamylase activities in the culture filtrates of strains 2M-43 and WU-2223L reached maximum levels of 3.62 U/ml and 2.11 U/ml, respectively, both at 3 d of cultivation, and thereafter decreased.

引用

页码：226 / 228

页数：3

共 15 条

[1] PRODUCTION OF THE RAW-STARCH DIGESTING AMYLASE OF ASPERGILLUS SP K-27 [J].

ABE, J ;

BERGMANN, FW ;

OBATA, K ;

HIZUKURI, S .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 27 (5-6) :447-450

[2] CITRIC-ACID FERMENTATION BY GAMMA-RAY INDUCED MUTANTS OF ASPERGILLUS-NIGER IN DIFFERENT CARBOHYDRATE MEDIA [J].

BEGUM, AA ;

CHOUDHURY, N ;

ISLAM, MS .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1990, 70 (04) :286-288

[3]

BIGELIS R, 1992, HDB APPLIED MYCOLOGY, V4, P357

[4]

KIRIMURA K, 1992, APPL MICROBIOL BIOT, V36, P573

[5] CITRIC-ACID PRODUCTION BY THE DIPLOID STRAINS OF ASPERGILLUS-NIGER OBTAINED BY PROTOPLAST FUSION [J].

KIRIMURA, K ;

NAKAJIMA, I ;

LEE, SP ;

KAWABE, S ;

USAMI, S .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 27 (5-6) :504-506

[6] ALTERATIONS OF RESPIRATORY SYSTEMS IN ASPERGILLUS-NIGER UNDER THE CONDITIONS OF CITRIC-ACID FERMENTATION [J].

KIRIMURA, K ;

HIROWATARI, Y ;

USAMI, S .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1987, 51 (05) :1299-1303

[7] IMPROVEMENT IN CITRIC-ACID PRODUCTION BY HAPLOIDIZATION OF ASPERGILLUS-NIGER DIPLOID STRAINS [J].

KIRIMURA, K ;

LEE, SP ;

NAKAJIMA, I ;

KAWABE, S ;

USAMI, S .

JOURNAL OF FERMENTATION TECHNOLOGY, 1988, 66 (04) :375-382

[8] INTRASPECIFIC PROTOPLAST FUSION OF CITRIC ACID-PRODUCING STRAINS OF ASPERGILLUS-NIGER [J].

KIRIMURA, K ;

YAGUCHI, T ;

USAMI, S .

JOURNAL OF FERMENTATION TECHNOLOGY, 1986, 64 (06) :473-479

[9] PHYSICAL MAPPING OF THE MITOCHONDRIAL-DNA FROM ASPERGILLUS-NIGER [J].

KIRIMURA, K ;

FUKUDA, S ;

ABE, H ;

KANAYAMA, S ;

USAMI, S .

FEMS MICROBIOLOGY LETTERS, 1992, 90 (03) :235-238

[10]

KUBICEK CP, 1986, CRIT REV BIOTECHNOL, V3, P331

← 1 2 →