SPECIES-SPECIFIC IDENTIFICATION OF AND DISTINCTION BETWEEN BORRELIA-BURGDORFERI GENOMIC GROUPS BY USING 16S RIBOSOMAL-RNA-DIRECTED OLIGONUCLEOTIDE PROBES

被引：60
作者：
MARCONI, RT
LUBKE, L
HAUGLUM, W
GARON, CF
机构：
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 03期
关键词：
D O I：
10.1128/JCM.30.3.628-632.1992
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cultures was used in slot blot analysis. Radiolabeled oligonucleotides designed to hybridize to specific 16S rRNA targets were used as probes. These probes allowed for both species-specific identification and genomic group typing of B. burgdorferi.
引用
收藏
页码：628 / 632
页数：5
相关论文
共 46 条
[41]   Identification of Lactobacillus alimentarius and Lactobacillus farciminis with 16S-23S rDNA intergenic spacer region polymorphism and PCR amplification using species-specific oligonucleotide [J].
Rachman, CN ;
Kabadjova, P ;
Prévost, H ;
Dousset, X .
JOURNAL OF APPLIED MICROBIOLOGY, 2003, 95 (06) :1207-1216
[42]   Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis [J].
Han, Hye Seung ;
Lee, Kyung-Yung ;
Lim, So Dug ;
Kim, Wan Seop ;
Hwang, Tae Sook .
ONCOLOGY LETTERS, 2010, 1 (03) :555-558
[43]   Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA [J].
Kwon, HS ;
Yang, EH ;
Yeon, SW ;
Kang, BH ;
Kim, TY .
FEMS MICROBIOLOGY LETTERS, 2004, 239 (02) :267-275
[44]   Rapid identification of potentially probiotic Bifidobacterium species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA [J].
Kwon, HS ;
Yang, EH ;
Lee, SH ;
Yeon, SW ;
Kang, BH ;
Kim, TY .
FEMS MICROBIOLOGY LETTERS, 2005, 250 (01) :55-62
[45]   THE USE OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES TO STUDY COMPETITION BETWEEN RUMINAL FIBROLYTIC BACTERIA - PURE-CULTURE STUDIES WITH CELLULOSE AND ALKALINE PEROXIDE-TREATED WHEAT-STRAW [J].
ODENYO, AA ;
MACKIE, RI ;
STAHL, DA ;
WHITE, BA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (10) :3697-3703
[46]   DEVELOPMENT OF POLYMERASE CHAIN-REACTION PRIMER SETS FOR DIAGNOSIS OF LYME-DISEASE AND FOR SPECIES-SPECIFIC IDENTIFICATION OF LYME-DISEASE ISOLATES BY 16S RIBOSOMAL-RNA SIGNATURE NUCLEOTIDE ANALYSIS (VOL 30, PG 2831, 1992) [J].
MARCONI, RT ;
GARON, CF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (04) :1026-1026
12345