ON ANALYSIS OF OPTICAL ROTATORY DISPERSION OF PROTEINS

被引：37
作者：
MAGAR, ME
机构：
[1] Department of Chemistry, University of Montana, Missoula
来源：
BIOCHEMISTRY | 1968年 / 7卷 / 02期
关键词：
D O I：
10.1021/bi00842a016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is described to analyze the optical rotatory dispersion of proteins and polypeptides in terms of linear combination of reference configurations. This is done by computing an approximation in the L2 norm. In this manner the percentage of various reference forms is found by a single computation instead of trial and error. While we have analyzed data for lysozyme and myoglobin in terms of the α helix, random coil, and β form, the method is capable in principle of analyzing data in terms of any number of reference configurations. If contributions from aromatic groups and disulfide bonds are precisely assessed, then they may be readily incorporated in the analysis. © 1968, American Chemical Society. All rights reserved.
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页码：617 / &
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