CHILD CEREBROSPINAL-FLUID ANALYSIS BY CAPILLARY ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE DETECTION

被引：70

作者：

NOUADJE, G

RUBIE, H

CHATELUT, E

CANAL, P

NERTZ, M

PUIG, P

COUDERC, F

机构：

[1] CNRS,BIOL MOLEC EUCARYOTE LAB,F-31062 TOULOUSE,FRANCE

[2] ZETA TECHNOL,F-31520 RAMONVILLE,FRANCE

[3] UNIV SCI PHARMACEUT,CHIM ANALYT & BROMATOL LAB,F-31062 TOULOUSE,FRANCE

[4] CTR HOSP REG PURPAN,SERV PEDIAT ONCOL,F-31300 TOULOUSE,FRANCE

[5] CTR CALUDIUS REGAUD,PHARMACOCINET LAB,F-31300 TOULOUSE,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 717卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00747-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was used to analyze a 50-mu 1 sample of cerebrospinal fluid from leukaemic children treated with high doses of methotrexate. Free amino acids and primary amines are labelled with fluorescein isothiocyanate prior to analysis. Electropherograms containing more than 50 peaks were obtained in less than 22 min. Twenty-one peaks were identified, and 19 were quantitated. Observed differences in individual amino acid levels are compared with healthy reference values. The results indicate that CE-LIF is useful as a selective, rapid and sensitive tool for the determination of free amino acids and amines in clinical biology studies.

引用

页码：293 / 298

页数：6

共 24 条

[1] Gelot, Ann. Biol. Clin., 45, (1987)
[2] Desgres, Boisson, Padieu, J. Chromatogr., 162, (1979)
[3] Huether, Amino Acids Avaiability and Brain Function in Health Disease, NATO Advance Science Institute Series, Series H Cell Biology, 20, (1988)
[4] Perry, Krieger, Hansen, Eisen, Ann. Neurol., 28, (1990)
[5] Kornhuber, Kornhuber, Hartmann, Kornhuber, Neurochem. Int., 12, (1988)
[6] Spink, Swann, Snead, Waniewski, Martin, Anal. Biochem., 158, (1986)
[7] Hagebfeld, Bjerkenstedt, Edman, Sedvall, Wiesel, J. Neurochem., 42, (1984)
[8] Hiraoka, Miura, Tominaga, Hattori, Clin. Biochem., 22, (1989)
[9] Tellez, Forges, Roussin, Hernandez, Monitoring excitatory amino acid release in vivo by microdialysis with capillary electrophoresis- electrochemistry, Journal of Chromatography A, 608, (1992)
[10] Chen, Liu, Hsieh, Stenberg, Clin. Chem., 37, (1991)

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