IMPROVEMENT OF THE THERMOSTABILITY OF BETA-GALACTOSIDASE FROM KLUYVEROMYCES-MARXIANUS

被引：0
作者：
TOMASKA, N
STREDANSKY, M
GEMEINER, P
STURDIK, E
机构：
[1] SLOVAK UNIV TECHNOL BRATISLAVA, DEPT BIOCHEM TECHNOL, BRATISLAVA 81237, SLOVAKIA
[2] SLOVAK ACAD SCI, INST CHEM, BRATISLAVA 84238, SLOVAKIA
来源：
PROCESS BIOCHEMISTRY | 1995年 / 30卷 / 07期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Comparative studies on some preparations of Kluyveromyces marxianus beta-galactosidase show that enzyme localized in permeabilized cells and in glutaraldehyde treated permeabilized cells was more thermostable at 45 degrees C, than cell-free enzyme, In contrast, the temperature optimum (50-52 degrees C) was not affected significantly by the intracellular localization Permeabilized cells immobilized by entrapment into hardened calcium alginate gel or treated with glutaraldehyde were used for repeated lactose hydrolysis at 45 degrees C and showed good operational stability.
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页码：649 / 652
页数：4
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